Fungichromin production by Streptomyces sp. WP-1, an endophyte from Pinus dabeshanensis, and its antifungal activity against Fusarium oxysporum.
Identifieur interne : 000193 ( Main/Exploration ); précédent : 000192; suivant : 000194Fungichromin production by Streptomyces sp. WP-1, an endophyte from Pinus dabeshanensis, and its antifungal activity against Fusarium oxysporum.
Auteurs : Cheng Peng [République populaire de Chine] ; Duopeng An [République populaire de Chine] ; Wen-Xin Ding [République populaire de Chine] ; Yuan-Xing Zhu [République populaire de Chine] ; Li Ye [République populaire de Chine] ; Jiyang Li [République populaire de Chine]Source :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2020.
Abstract
In this study, we isolated an endophytic Streptomyces sp. strain, WP-1, from surface-sterilized barks of Pinus dabeshanensis, an endangered Chinese plant. WP-1 showed strong antifungal activity against diverse pathogenic fungi, such as Fusarium oxysporum, Rhizoctonia solani, Phytophthora infestan, and Candida albicans. Based on phylogenetic analyses, preliminary identification suggested that the WP-1 strain belonged to the genus Streptomyces. Column chromatogram and HPLC were employed to isolate the primary antifungal component from the culture medium of WP-1, and it was identified as the methylpentaene macrolide antibiotic, fungichromin (FC). In this study, for the first time, using in vitro bioassay studies, we revealed that FC strongly inhibited mycelial growth and conidia germination in Fusarium oxysporum. The median inhibitory concentration of FC was found to be 3.80 mg/L. The fermentation conditions of the WP-1 strain were further investigated to improve FC production. We found that supplementation of the synthetic medium with oils (soybean oil, oleic acid, and so on), particularly during the initial stage of fermentation, significantly increased the FC yield. Ammonium-trapping agent (magnesium phosphate) was used as an additive to increase FC yield to 5741.7 mg/L. It was 2.9-fold more as compared to the highest FC yield reported so far where Streptomyces padanus PMS-702 was used for FC production. KEY POINTS: • Isolation and identification of a fungichromin-producing endophytic actinomycete WP-1 strain. • Fungichromin production was significantly improved via oils and ammonium-trapping agents addition. • Discovery of the antifungal activity of fungichromin against Fusarium oxysporum.
DOI: 10.1007/s00253-020-10996-z
PubMed: 33170328
Affiliations:
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WP-1, an endophyte from Pinus dabeshanensis, and its antifungal activity against Fusarium oxysporum.</title><author><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="An, Duopeng" sort="An, Duopeng" uniqKey="An D" first="Duopeng" last="An">Duopeng An</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Ding, Wen Xin" sort="Ding, Wen Xin" uniqKey="Ding W" first="Wen-Xin" last="Ding">Wen-Xin Ding</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Zhu, Yuan Xing" sort="Zhu, Yuan Xing" uniqKey="Zhu Y" first="Yuan-Xing" last="Zhu">Yuan-Xing Zhu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Ye, Li" sort="Ye, Li" uniqKey="Ye L" first="Li" last="Ye">Li Ye</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Li, Jiyang" sort="Li, Jiyang" uniqKey="Li J" first="Jiyang" last="Li">Jiyang Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China. lijiyang@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33170328</idno><idno type="pmid">33170328</idno><idno type="doi">10.1007/s00253-020-10996-z</idno><idno type="wicri:Area/Main/Corpus">000011</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000011</idno><idno type="wicri:Area/Main/Curation">000011</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000011</idno><idno type="wicri:Area/Main/Exploration">000011</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fungichromin production by Streptomyces sp. WP-1, an endophyte from Pinus dabeshanensis, and its antifungal activity against Fusarium oxysporum.</title><author><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="An, Duopeng" sort="An, Duopeng" uniqKey="An D" first="Duopeng" last="An">Duopeng An</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Ding, Wen Xin" sort="Ding, Wen Xin" uniqKey="Ding W" first="Wen-Xin" last="Ding">Wen-Xin Ding</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Zhu, Yuan Xing" sort="Zhu, Yuan Xing" uniqKey="Zhu Y" first="Yuan-Xing" last="Zhu">Yuan-Xing Zhu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Ye, Li" sort="Ye, Li" uniqKey="Ye L" first="Li" last="Ye">Li Ye</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author><author><name sortKey="Li, Jiyang" sort="Li, Jiyang" uniqKey="Li J" first="Jiyang" last="Li">Jiyang Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China. lijiyang@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203</wicri:regionArea><wicri:noRegion>201203</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="eISSN">1432-0614</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, we isolated an endophytic Streptomyces sp. strain, WP-1, from surface-sterilized barks of Pinus dabeshanensis, an endangered Chinese plant. WP-1 showed strong antifungal activity against diverse pathogenic fungi, such as Fusarium oxysporum, Rhizoctonia solani, Phytophthora infestan, and Candida albicans. Based on phylogenetic analyses, preliminary identification suggested that the WP-1 strain belonged to the genus Streptomyces. Column chromatogram and HPLC were employed to isolate the primary antifungal component from the culture medium of WP-1, and it was identified as the methylpentaene macrolide antibiotic, fungichromin (FC). In this study, for the first time, using in vitro bioassay studies, we revealed that FC strongly inhibited mycelial growth and conidia germination in Fusarium oxysporum. The median inhibitory concentration of FC was found to be 3.80 mg/L. The fermentation conditions of the WP-1 strain were further investigated to improve FC production. We found that supplementation of the synthetic medium with oils (soybean oil, oleic acid, and so on), particularly during the initial stage of fermentation, significantly increased the FC yield. Ammonium-trapping agent (magnesium phosphate) was used as an additive to increase FC yield to 5741.7 mg/L. It was 2.9-fold more as compared to the highest FC yield reported so far where Streptomyces padanus PMS-702 was used for FC production. KEY POINTS: • Isolation and identification of a fungichromin-producing endophytic actinomycete WP-1 strain. • Fungichromin production was significantly improved via oils and ammonium-trapping agents addition. • Discovery of the antifungal activity of fungichromin against Fusarium oxysporum.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">33170328</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><Issue>24</Issue><PubDate><Year>2020</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Fungichromin production by Streptomyces sp. WP-1, an endophyte from Pinus dabeshanensis, and its antifungal activity against Fusarium oxysporum.</ArticleTitle><Pagination><MedlinePgn>10437-10449</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-020-10996-z</ELocationID><Abstract><AbstractText>In this study, we isolated an endophytic Streptomyces sp. strain, WP-1, from surface-sterilized barks of Pinus dabeshanensis, an endangered Chinese plant. WP-1 showed strong antifungal activity against diverse pathogenic fungi, such as Fusarium oxysporum, Rhizoctonia solani, Phytophthora infestan, and Candida albicans. Based on phylogenetic analyses, preliminary identification suggested that the WP-1 strain belonged to the genus Streptomyces. Column chromatogram and HPLC were employed to isolate the primary antifungal component from the culture medium of WP-1, and it was identified as the methylpentaene macrolide antibiotic, fungichromin (FC). In this study, for the first time, using in vitro bioassay studies, we revealed that FC strongly inhibited mycelial growth and conidia germination in Fusarium oxysporum. The median inhibitory concentration of FC was found to be 3.80 mg/L. The fermentation conditions of the WP-1 strain were further investigated to improve FC production. We found that supplementation of the synthetic medium with oils (soybean oil, oleic acid, and so on), particularly during the initial stage of fermentation, significantly increased the FC yield. Ammonium-trapping agent (magnesium phosphate) was used as an additive to increase FC yield to 5741.7 mg/L. It was 2.9-fold more as compared to the highest FC yield reported so far where Streptomyces padanus PMS-702 was used for FC production. KEY POINTS: • Isolation and identification of a fungichromin-producing endophytic actinomycete WP-1 strain. • Fungichromin production was significantly improved via oils and ammonium-trapping agents addition. • Discovery of the antifungal activity of fungichromin against Fusarium oxysporum.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Cheng</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>An</LastName><ForeName>Duopeng</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Wen-Xin</ForeName><Initials>WX</Initials><AffiliationInfo><Affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Yuan-Xing</ForeName><Initials>YX</Initials><AffiliationInfo><Affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Li</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jiyang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai, 201203, China. lijiyang@fudan.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antifungal fermentation</Keyword><Keyword MajorTopicYN="N">Endophytic actinomycete</Keyword><Keyword MajorTopicYN="N">Fungichromin production</Keyword><Keyword MajorTopicYN="N">Polyene macrolides</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>10</Day><Hour>12</Hour><Minute>13</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33170328</ArticleId><ArticleId IdType="doi">10.1007/s00253-020-10996-z</ArticleId><ArticleId IdType="pii">10.1007/s00253-020-10996-z</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name></noRegion><name sortKey="An, Duopeng" sort="An, Duopeng" uniqKey="An D" first="Duopeng" last="An">Duopeng An</name><name sortKey="Ding, Wen Xin" sort="Ding, Wen Xin" uniqKey="Ding W" first="Wen-Xin" last="Ding">Wen-Xin Ding</name><name sortKey="Li, Jiyang" sort="Li, Jiyang" uniqKey="Li J" first="Jiyang" last="Li">Jiyang Li</name><name sortKey="Ye, Li" sort="Ye, Li" uniqKey="Ye L" first="Li" last="Ye">Li Ye</name><name sortKey="Zhu, Yuan Xing" sort="Zhu, Yuan Xing" uniqKey="Zhu Y" first="Yuan-Xing" last="Zhu">Yuan-Xing Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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